P12.13.B Investigating the tumor - immune cell crosstalk in<i>ex vivo</i> glioblastoma models
نویسندگان
چکیده
Abstract Background Immunotherapy is a promising therapeutic approach to fight cancer by activating the immune system. Multiple immune-based strategies are under development that aim at recruiting or re-activating cellular components of While immunotherapies have recently revolutionized therapy, they shown so far little success in glioblastoma patients. To enhance efficacy novel strategies, we need better understand immunogenic status cells and their cross-talk with different microenvironmental niches. Material Methods We assessed expression molecules related antigen processing presentation as well checkpoints patient tumor databases series patient-derived organoids, 3D stem-like cultures adherent cell lines varying conditions (varying oxygen levels, inflammation). further established an allogenic co-culture protocol for organoids isolated from HLA matched donor blood, allowing functional assessment crosstalk between cells. Results Analysis large cohort tumors preclinical models shows inter-patient heterogeneity level major histocompatibility complex (MHC)-MHC-II, checkpoints. Glioblastoma general express MHC-I machinery, albeit levels. MHC-II variably expressed across Different microenvironment conditions, including hypoxia interferon-γ, impact immune-related molecules. Upon co-culture, HLA-matched donor-derived T integrate into core display reciprocal Conclusion Assessing responses key improve patient-specific immunotherapies. Advanced incorporating compartment appear clinically-relevant ex vivo studies.
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ژورنال
عنوان ژورنال: Neuro-oncology
سال: 2022
ISSN: ['1523-5866', '1522-8517']
DOI: https://doi.org/10.1093/neuonc/noac174.278